Use the diagram of a microscope provided to identify the parts of the microscope. Use the scanning 4x objective and course focus adjustment to focus, then move the mechanical stage around to find the letter e. Looking through the ocular, use a slight turn of the fine adjustment knob to focus the image of the letter e. Now that you have determined the size of the fields on your microscope, place the slide with the letter e back on the stage. Obtain a prepared slide of the letter e or make your own slide by cutting out an e from the newsprint provided. When you move the slide to the left, in what direction does the letter e appear to move. Create a wet mount slide of the letter e as indicated on the back of this sheet. How to prepare a microscope slide to zoom in on a specimen. Compound microscope glass slide with the letter e on it results. To observe and draw the letter e as it appears under a microscope. Describe the position of the image of the letter e through the microscope compared to.
Write out the rule for determining total magnification of a compound microscope. Take the letter e slide and clean it with a kim wipe tissue. Use the coarse focus knob and raise the stage until it is in its highest. Draw the letter e exactly as it looks under low power. Get a microscope from the cabinet below your lab bench, being sure to handle it by the arm and base refer to image on page 2, and place it on the bench in front of you.
The virtual microscope loads the letter e slide at the center of the stage and starts viewing at 4x scanning power objective lens. I followed the instructions to plug in the microscope and pick up the letter e slide that was sitting in a tray next to the microscope. To learn how to use the microscope procedure part 1. Introduction to the microscope lab activity beachwood city schools. Cut out the letter e and place it on the slide face up. Familiarize yourself with the parts of the microscope. This includes a list of the materials needed to mount slides, an explanation of the different techniques of mounting slides and when to use them, what techniques to use for the best results depending upon the specimen, and which style of slides to choose for which type of observations youll be making. The directions in the lab handout said to place the letter e on the slide so that the e was right side up and to ake sure the letter was positioned directly over the hole where the light was. Lab exercise 2 bluegrass community and technical college. Make sure that while sitting in thechair the letter e still appears as a correctly oriented readable e. Note the orientation when viewed through the oculars.
Use a dropper to place one drop of water on the piece of newspaper. If you do not know how to use a microscope properly, you will. With your scissors cut out the letter e from the newsprint. Clean a microscope slide and prepare a wet mount of the letter, using the procedure described below. L4 microscope letter e and drawing student laboratory packet. Next make sure the stage is completely turned all the way down using the coarse focus knob and always begin.
You should now be able to label and understand each part of the compound microscope. How does the letter e as seen through the microscope differ from the way an e normally appears. Place it on a slide and cover the letter e with a cover slip. What are the numbers listed on each of the objectives.
We will be using a compound light microscope in this lab to view various cells and tissues. To view something with your microscope, place the slide under the stage clips. Calculate the high power magnification of this microscope. Oct 21, 2019 this article serves as a simple, easytofollow guide on how to prepare a microscope slide. Cut out a small colored letter e out of a magazine or paper. In previous lessons, you have learned how to properly use a microscope and care for a microscope. Preparing and examining a wet mount for the letter e read each of the following steps carefully and place a check on each line when you have finished each step 1.
Place the cut out e on a glass microscope slide and slowly lower a coverslip over it to hold it in place. Microscope powerpoint, worksheets that reinforce, quiz and test to assess and the classic micro e lab to familiarize students with the use of the microscope. After going over some rules for the microscope we set it up. Be sure to note the orientation of the letter e as it appears to your naked eye. On the diagram label the microscope parts and write what they do. Pull the stage clip back and place the slide in the slide holder. How does the position of the letter viewed through the microscope differ from the actual. Letter e look through a newspaper for the smallest letter e you can find.
Draw the image you see of the letter e or part of it on high power. What is the estimated size of the letter e in millimeters. This labs objective is to attain proficiency in using microscopes. L4 microscope letter e and drawing page 3 of 9 e 4. Microscope lab conclusion in this lab we used a compound light microscope to study and inspect multiple items such as the letter e in newspaper clippings, a ruler, strands of thread, and other items such as camel hair and a dragonfly wing. Use the coarse adjustment knob to gently raise the stage until the scanningpower objective is as close to the slide as it will go. Microscopes are tools used to enlarge images of small objects so as they can be studied. Move it and record the changes in light intensity as. Follow the tutorial and use the microscope adjustments to focus the e on each of the objectives. In the lab version, students would compare onion cells to their own cheek. Cut out the letter e from the newspaper and place it on the glass slide facing up. Turn on the microscope lamp and set the light intensity at 8. Before coming to lab read this laboratory exercise and pp.
Place the letter e slide in the stage clips slide label right side up. The parts of the microscope objective lens total magnification 4x 10x 40x procedure 1. Cut a piece of newsprint that contains the letter e. Oneill will use the letter e to teach a few valuable lessons about how to operate a compound light microscopecheck out this link to a free. This virtual lab also starts with the letter e and then has students look at plant cells and human blood and make comparisons. Wet mount microscope lab preparing a wet mount of the letter e. Using the 4x objective lens and the coarse adjustment, focus on the letter e again. Adjust the iris diaphragm until you have the proper amount of light with good.
Cut out a letter e from a newspaper or other printed page. Place the letter in the center of a microscope slide. Place the slide on a white piece of paper find the specimen the letter e on the slide. You will see a blurry image within the field of view. If you do not know how to use a microscope properly, you will have a difficult time finding specimenscells in this unit. Introduction to the microscope lab activity denton isd. Place the cover slip on top of the e and drop of water at a 45degree angle and lower. Read the page at your station titled calculating total magnification and fill in the notes below. In the circle to the right, draw the letter e in the same size and position as you see it in the microscope. However, there will be a quiz on microscope care at the beginning of lab.
Place the slide on the stage in the slide holder so that the letter e is centered over the light beam passing through the stage. Make sure the bottom of the slide is dry before you place it on the stage. We all know that an e is round with a line connecting the upper half. Using your eyedropper, place a drop of water on the edge of the cover slip where it touches the. Adjust the condenser to its highest position and switch on the light source of your microscope. Jul 04, 2020 generally, i have my students practice with real microscopes, starting with a basic tutorial lab where they focus on the letter e. The compound light microscope is an instrument containing two lenses, which magnifies, and a variety of knobs to resolve focus the picture.
Thoroughly clean the stage and microscope slides to prevent damage to the microscope. The letter e is something everyone should be familiar with or at least i hope so. We created a chart on our ipads and cut out the letters, e, o, and c from the newspaper. In the appropriate place below, draw the letter e as seen through the microscope. If you missed the microscope lab we did in class, you will need to make it up by using a virtual microscope which can be accessed on the internet. You will also use a slightly different type of light microscope called a stereoscopic dissecting microscope.
Prepare an object for viewing under the microscope. Get the letter e in focus using the low power lens. Exercise 1a getting familiar with the microscope you will first get acquainted with the major parts of the compound light microscope before learning the proper way to use it. Place the slide of the letter e on the stage so that the letter is over the hole and is right side up. The other person in the group will pick up a slide with letter e. Center the wet mount on the stage with the letter e in its normal upright position. Oct 27, 2014 the first thing we did in this lab was learn some basic rules on how to take care of the microscope that were in the lab sheet. Turn the lowpower objective into position and bring the e into focus. Use the slide holder adjustment knobs to move the letter e over the light source. Move the slide slightly to the left while observing the letter e in the microscope. The virtual microscope is a little more complicated than the microscope we used in the lab, but with patience, you should be able to complete this activity. The compound light microscope, which is going to be used in this lab activity, is an instrument with two lenses and various knobs to focus the image.
A microscope has a 20 x ocular eyepiece and two objec ives of 10 and 43 x respectively. How does the ink appear under the microscope compared to normal view. Today, you will use that knowledge to determine how a microscope works. In three steps using complete sentences, describe how to make a proper wet mount of the letter e. In this lab, we will learn about the proper use and handling of the microscope. Use your mouse to adjust the coarse focus until the image is in focus. Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope. Draw the e exactly as it appears scanning 4 low 10 high 100 3. Now slowly move the stage down by turning the coarse adjustment knob until the letters come into focus. Draw the letter e exactly as it looks under medium power. Properly use and focus a microscope from a low power to a high power objective lens.
Lab 3 use of the microscope introduction in this laboratory you will be learning how to use one of the most important tools in biology the compound light microscope to view a variety of specimens. Letter e lab introduction micro refers to tiny, scope refers to view or look at. We will use microscopes to observe specimens by preparing wet mounts. Focus the slide first on low power, using the coarse and fine focus, then increase the power and refocus only using the fine focus knob. Place the letter e slide onto the mechanical stage. Place the letter on the center of a glass microscope slide and add a. Determine how the lens system of a microscope changes the specimens position. What is the total magnification when observing a specimen under the low objective.
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